Lab Basics

Preliminaries

Dilutions

Mixtures and solutions

Molarity

Solutions

Bacteria and plasmids

Growing Bacteria

Culturing Anaerobic Bacteria

Transformation of Chemically Competent E.coli

DNA Preparation

Ammonium Acetate DNA Precipitation

Minipreps

Maxipreps

PCR

Oligo calculator – use the salt adjusted Tm for PCR reactions

Reverse complement calculator

PCR Methods

PCR polymerase compendium

Colony PCR

SYBR Green  RT-PCR Protocol

Restriction Analysis

Restriction Digests

NEB Enzyme Finder – Find restriction enzymes by sequence, name, or type

Cloning and Gene Assembly

Cloning 1

Cloning 2

Ligation

Gibson Assembly® Cloning (NEB)

Topo TA Cloning

DNA Assembly of Oligonucleotides via PCR

BioBrick Assembly

Gateway Cloning (via site specific recombination)

Chitin Impact™ Kit Manual (NEB)

DNA Sequence Analysis

Using GENtle and BLAST

Proteins

Westerns

Western Protocol

Chitin Beads Protein Purification

HisPur™ Cobalt Resin Protein Purification Protocol

SuperSignal™ West Pico Chemiluminescent Substrate Protocol (Pierce)

Cell Culture

Tissue Culture Basics

Transfection

Calcium chloride transfection of mammalian cells (using CalPhos kit)

General Lab Methods

Cleaning Electroporation Cuvettes

Regeneration of DNA Silica Columns

Solution Recipes

SYBR Green RT-PCR Protocol

 

Lab Miscellaneous

Antibiotic Concentrations for Bacterial Selection (Calbiochem)

Useful Tools

NEBioCalculator – DNA ligation calculator, DNA & RNA mass-mole calculator, dilution calculator, molarity calculator, OD260 calculator

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